This in-depth lecture and hands-on laboratory workshop (wet lab & in silica) is ideal for those research and bench scientists who are interested in a comprehensive introduction to single cell RNA-Seq. The core of this workshop is composed of highlighting key aspects of NSG and RNA-seq methodologies, with subsequent introduction into the modern armamentarium of tools to conduct these experiments. Further emphasis will be placed on such important aspects as sample preparation, quality control validation and enrichment as well as extensive use of different single cell RNA-Seq data analysis tools (Seurat, Monocle, Pseudo-time Analysis, Clustering Analysis in-depth: t-SNE and Principal Component Analysis).